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. 2021 Oct 7;12:5864. doi: 10.1038/s41467-021-26083-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Schematic representation of the targeted Rn7sk alleles in the two mouse lines generated. loxP sites = black triangles; TATA = TATA box; PSE = proximal sequence elements; DSE = distal sequence elements. b Schematic representation of the transgenes used to delete Rn7sk within the interfollicular epidermis (IFE). c Treatment regime of the experiment shown in (d). d Rn7sk RNA in situ hybridization (brown; top panel), haematoxylin and eosin staining (H&E, middle panel) and KRT10 (K10; green) and KRT14 (K14; red) immunofluorescence (bottom panel) of control (CTR) and Rn7sk knock-out (Rn7sk cKO) mice. e, f Quantification of skin area with reduced (red) (e) or increased (blue) (f) epidermal cellularity at the indicated time points (n = mice). g Treatment regime of the experiment shown in (h, i). h H&E staining (top panel) and KRT10 (K10; green) and KRT14 (K14; red) immunofluorescence (bottom panel) of mice treated with 4-hydroxytamoxifen (4-OHT) as shown in (g). DAPI (blue): nuclear counterstain (d, h). i Quantification of skin area with decreased (red; left panel) or increased (blue; right panel) epidermal cellularity in mice treated as shown in (g) (n = mice). J Graphical summary of the data shown in (e, f, i). Mouse line 1 was used in (d, h, i). Pooled data from mouse lines 1 and 2 are shown in (e, f). Scale bar: 10 μm (d, h). Shown is mean. Multiple two-tailed unpaired t-tests. Exact p-values are indicated. Source data are provided as a Source Data file.