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. 2021 Oct 7;11:19991. doi: 10.1038/s41598-021-99528-x

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© The Author(s) 2021, corrected publication 2024

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Microscopy of I. sakaiensis grown on poly(ethylene terephthalate) (PET). (a) Bright field and fluorescence microscopy of Nile red-stained I. sakaiensis cells after growth on PET for 0 (pre-cultured cells in 802 nutrient-rich medium for 1 day), 3, 6, and 9 days. Box-and-whisker plots show the distribution of cell areas (b) and average fluorescence intensity of Nile red in cells (c); median values, 25th to 75th percentiles, and range are shown. n, number of cells or fluorescent dot assemblies. ND, not detected. Different letters above the plots indicate significant differences among groups (Tukey's multiple comparisons test, P < 0.0001). (d) Transmission electron microscopy image of cells grown on PET granules for 6 days. Scale bar, 600 nm.