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. 2021 Oct 7;11:19957. doi: 10.1038/s41598-021-99266-0

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Activation of Supraoptic and Paraventricular hypothalamic nucleus by food intake. Number (mean ± SEM) of single OT (a, d), percentage of OT expressing c-Fos (b, e) and percentage of single c-Fos (c, f) cells in the SON (a–c) and PVNm (d–f) in rats kept ad libitum (AL), under restricted food access (RF), under RF followed by 2 days of fasting (RF-F) and ad libitum fed followed by 2 days of fasting (AL-F) at four different zeitgeber times (n = 5 per time point). Horizontal stripped bar represents mealtime (from ZT5 to ZT7) for the RF group and expected mealtime for the RF-F group. **Indicate difference between the highest and lowest value within the same group (P < 0.05); ++indicate difference between groups at the same time point (P < 0.05).