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. 2021 Oct 7;4:1158. doi: 10.1038/s42003-021-02688-9

Fig. 2. MO knock-down of enpp4 expression disrupts pronephros formation.

Fig. 2

Embryos targeted with 10 ng of enpp4 MO1 or 10 ng of both enpp4 MOs (c, n) and 250 pg of lacZ mRNA were examined by 3G8/4A6 antibody staining (a–c) or whole-mount in situ hybridization with the following probes: slc5a1.1 (d), slc12a1 (e, f), clcnkb (g) and gata3 (h) at stage 37/38; wt1 (i) and nphs1 (j) at stage 33/34, lhx1 (k–n) at stages 28, 24 and 14, pax8 (o) at stage 14. The embryo shown in (f) was co-injected with 2 ng of mouse Enpp4 mRNA to rescue enpp4 knock-down phenotype. The asterisk denotes the control, uninjected side of each embryo. Blank arrowheads in (h) indicate the anterior limit of gata3 expression. See also Supplementary Table 2 for raw data and statistical analyses and Supplementary Figs. 2 and 3.