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. 2021 Oct 7;4:1158. doi: 10.1038/s42003-021-02688-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — a CHO cells were transfected with Xlenpp4-pcDNA3.1 or empty vector (control) and the cellular distribution of Enpp4 determined by western blotting using anti-XlEnpp4 antibody and proteins extracts from membrane (mb), soluble (sb) or whole cells (wh) fractions. b Representative images of the cellular distribution of Enpp4 determined by immunofluorescence using anti-XlEnpp4 or anti-mEnpp4 antibodies from CHO cells transfected with Xlenpp4-pcDNA3.1, Xlenpp4T72S-pcDNA3.1, mEnpp4-pcDNA3.1 or empty pcDNA3.1 vector (control). Corresponding brightfield images are also presented. Scale bar represents 20 µm.