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. 2021 Oct 7;4:1158. doi: 10.1038/s42003-021-02688-9

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — Injected embryos were examined by 3G8/4A6 antibody staining following a, b double targeted injection of 2 ng s1pr5.l mRNA and 1 ng of enpp4 mRNA, c single targeted injection of s1pr5.l, d enpp4 mRNA or e, f embryos injected with 15 ng of s1pr5.L MO. g, h Double targeted injection of 7.5 ng of s1pr5.L MO and 5 ng of enpp4 MO1. i, j Single targeted injection of s1pr5.L MO (i) or enpp4 MO1 (j). k, l Double targeted injection of 15 ng of s1pr5.L MO and 2 ng of enpp4 mRNA (k) and 15 ng of cMO and 2 ng of enpp4 mRNA (l). An asterisk denotes the control uninjected side. An arrowhead indicates ectopic 3G8 staining (see also Supplementary Table 5 and Supplementary Figs. 6 and 7).