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. 2021 Oct 7;4:1158. doi: 10.1038/s42003-021-02688-9

Fig. 7. Enpp4 specifically binds to the lysophospholipid, phosphatidylserine.

Fig. 7

a Membrane Lipid stripTM was incubated with membrane protein extracts from Enpp4 overexpressing CHO cells and the bound Enpp4 protein detected with anti-XlEnpp4 serum. b, c Nitrocellulose membranes were spotted with increasing amount of PA or PS and incubated with membrane protein extracts from CHO cells transfected with enpp4-pcDNA3.1, with enpp4T72S-pcDNA3.1 or empty plasmid (control) and the bound proteins detected with anti-XlEnpp4 serum. LPA lysophosphatidic acid, LPC lysophosphocholine, PA phosphatidic acid, PC phospatidylcholine, PE phosphatidylethanolamine, PS phosphatidylserine, PtIns phosphatidylinositol, S1P shingosine-1-phosphate (see also Supplementary Fig. 8).