Fig. 6. Exogenous overexpression of PCK1 augments pancreatic cancer cell growth and motility.

Established pancreatic cancer cell lines (PANC-1 and PATU-8988) (A–F) or the primary pancreatic cancer cells (“pPC1/2/3”) (G–I) with a lentiviral construct encoding the PCK1 cDNA (“OE-PCK1”) or the empty vector (“Vec”), were established and cultured for applied time periods; Expression of listed mRNA and proteins were tested by qRT-PCR and Western blotting assays (A–C, F, G); Cell proliferation (EdU-positive nuclei ratio, D, H), migration and invasion (“Transwell” assays, I) were tested by the listed assays, with data quantified. Data were presented as mean ± standard deviation (SD, n = 5). *P < 0.05 vs. “Vec” cells. “n.s.” stands for non-statistical difference (B). The experiments were repeated five times with similar results obtained. Scale bar = 100 μm (D, E).