Fig. 7. PCK1 silencing suppresses pancreatic cancer xenograft growth in mice.

PANC-1 xenografts-bearing female BALB/c nude mice were established by subcutaneous injection of PANC-1 cells expressing PCK1 shRNA (“sh-PCK1-seq1”) or scramble control shRNA lentivirus (“shC”); Tumor volumes (A) and mice body weights (E) were recorded every five days. After 25 days, all tumors were separated (B, C) and tumor weights were recorded (D). In tumor tissue lysates expressions of PCK1 mRNA (F) and listed proteins (G, H) were tested by qRT-PCR and Western blotting assays, with results quantified. Data were presented as mean ± standard deviation (SD). *P < 0.05 vs. “shC” tumors.