Figure 5. Increased expression of IFI44 and Mx1 in the PFC of depressed subjects.
(A) Increase in IFN-I stimulated genes (IFI44 and Mx1), complement component (C3) and M1 phenotyppe markers in the PFC of depressed suicide subjects. mRNA levels of these genes in the PFC of depressed suicide (n=15) and control (n=15) subjects were measured by qRT-PCR. mRNA levels were normalized to housekeeping genes 18S and B2m. C3 (***p <0.001), IFI44 (***p <0.001), Mx1 (***p <0.001), CD16 (***p <0.001), iNOS (*p <0.05), Cx3CR1 (*p <0.005) and TNFα (***p =0.001) as compared to the controls, multivariate analysis of variance (MANOVA). (B-E) Representative graphs showing correlation between the mRNA levels. B, Correlation between IFI44 and C3 (Pearson r =0.72; p<0.0001; R2 = 0.5147). C, Correlation between IFI44 and CD16 (Pearson r =0.69; p<0.0001; R2 = 0.4822). D, Correlation between IFI44 and TNFα (Pearson r =0.86; p<0.0001; R2 = 0.7332). E, Correlation between Mx1 and TNFα (Pearson r =0.70; p<0.0001; R2 = 0.4906).