TABLE 1.
Released N-glycans identified from CHO-Spike protein.
Experimental Mass [M + Na]+ | Theoretical Mass [M + Na]+ | Delta Mass | Composition | Relative percentage |
---|---|---|---|---|
1136.610 | 1136.40 | 0.209 | N3H3 | 0.56% |
1257.570 | 1257.43 | 0.142 | N2H5 | 2.04% |
1282.621 | 1282.46 | 0.161 | N3H3F1 | 0.58% |
1298.591 | 1298.45 | 0.137 | N3H4 | 0.77% |
1419.630 | 1419.48 | 0.149 | N2H6 | 1.86% |
1444.633 | 1444.51 | 0.121 | N3H4F1 | 0.79% |
1460.649 | 1460.51 | 0.142 | N3H5 | 0.71% |
1485.683 | 1485.54 | 0.145 | N4H3F1 | 1.22% |
1501.680 | 1501.53 | 0.146 | N4H4 | 1.36% |
1581.713 | 1581.53 | 0.179 | N2H7 | 0.93% |
1588.703 | 1588.57 | 0.137 | S(23)1-N3H4 | 0.37% |
1647.757 | 1647.59 | 0.165 | N4H4F1 | 4.00% |
1663.759 | 1663.59 | 0.172 | N4H5 | 7.09% |
1743.668 | 1743.59 | 0.082 | N2H8 | 1.41% |
1750.754 | 1750.62 | 0.135 | S(23)1-N3H5 | 0.62% |
1791.775 | 1791.65 | 0.129 | S(23)1-N4H4 | 0.85% |
1809.834 | 1809.64 | 0.190 | N4H5F1 | 22.89% |
1825.837 | 1825.64 | 0.198 | N4H6 | 0.77% |
1850.848 | 1850.67 | 0.177 | N5H4F1 | 0.47% |
1905.804 | 1905.64 | 0.165 | N2H9 | 0.56% |
1930.790 | 1930.67 | 0.120 | N3H7F1 | 1.78% |
1937.910 | 1937.70 | 0.207 | S(23)1-N4H4F1 | 2.21% |
1953.904 | 1953.70 | 0.206 | S(23)1-N4H5 | 4.43% |
2012.927 | 2012.72 | 0.203 | N5H5F1 | 1.38% |
2028.911 | 2028.72 | 0.193 | N5H6 | 0.88% |
2099.985 | 2099.76 | 0.229 | S(23)1-N4H5F1 | 19.53% |
2115.973 | 2115.75 | 0.222 | S(23)1-N4H6 | 0.79% |
2175.009 | 2174.78 | 0.232 | N5H6F1 | 3.39% |
2244.063 | 2243.81 | 0.253 | S(23)2-N4H5 | 0.54% |
2303.057 | 2302.84 | 0.222 | S(23)1-N5H5F1 | 0.69% |
2319.111 | 2318.83 | 0.281 | S(23)1-N5H6 | 0.65% |
2378.122 | 2377.86 | 0.266 | N6H6F1 | 0.37% |
2390.136 | 2389.87 | 0.268 | S(23)2-N4H5F1 | 3.51% |
2465.166 | 2464.89 | 0.278 | S(23)1-N5H6F1 | 2.60% |
2540.199 | 2539.91 | 0.290 | N6H7F1 | 0.79% |
2593.117 | 2592.95 | 0.170 | S(23)2-N5H5F1 | 0.27% |
2609.265 | 2608.94 | 0.323 | S(23)2-N5H6 | 0.32% |
2668.270 | 2667.97 | 0.303 | S(23)1-N6H6F1 | 0.22% |
2755.329 | 2755.00 | 0.329 | S(23)2-N5H6F1 | 2.17% |
2830.358 | 2830.02 | 0.338 | S(23)1-N6H7F1 | 0.87% |
3045.463 | 3045.11 | 0.352 | S(23)3-N5H6F1 | 0.72% |
3120.504 | 3120.13 | 0.372 | S(23)2-N6H7F1 | 0.97% |
3410.492 | 3410.24 | 0.249 | S(23)3-N6H7F1 | 0.64% |
3700.758 | 3700.35 | 0.403 | S(23)4-N6H7F1 | 0.42% |