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. 2021 Sep 24;11:721644. doi: 10.3389/fonc.2021.721644

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Copyright © 2021 Cai, Zhang, Hu, Hu, Yang, Qiu and Chu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Silencing of CDT1 inhibited the proliferation, migration and invasion of HCC cells. (A) Western blot detection of CDT1 expression after knockdown of CDT1 in liver cancer cells. (B) The effect of CDT1 knockdown on cell viability at 24, 48, 72 and 96h after seeding in plates was measured by CCK-8 assay. (C–E) Transwell analysis and wound healing assay reflected the migration ability of LM3 and Hep3B cell lines. (F) Images of the colony formation assay after knockdown of CDT1 in HCC cells (G, H) Relative quantification of the colony areas is shown. Scale bars in (F) equal 5mm. Data are expressed as means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001.