FIGURE 4.

Biophysical characterization of SERBP1 oligomer state in solution. Size exclusion chromatography profiles of (A) SERBP1 189–400 and (B) SERBP1 149–400, respectively, in the absence (solid) and presence (red) of 2 M GdnHCl recorded at A₂₈₀. (C) Size distribution histograms of the apparent hydrodynamic radius (R _h) derived from dynamic light scattering experiments of SERBP1 189–400 in the absence (solid) and presence (dashed) of 2 M GdnHCl. (D) Analytical ultracentrifugation sedimentation velocity coefficient profiles of SERBP1 189–400 in the absence (solid) and presence (dashed) of 2 M GdnHCl.