Skip to main content
. 2021 Sep 24;12:716684. doi: 10.3389/fphar.2021.716684

FIGURE 2.

FIGURE 2

Hig prevented RSC96 and DRG neuron from oxidative stress damage. (A) RSC96 intercellular level of ROS stained by DCFH-DA dye was observed under the microscope. Scar bar = 200 µm. (B) RSC96 intercellular level of ROS stained by DCFH-DA dye was observed by flow cytometer. (C) The ratios of RSC96 intercellular ROS level compared with the control group were calculated. (D) Quantification of RSC96 intercellular MDA level in different groups. (E) Quantification of RSC96 intercellular SOD level in different groups. (F) Quantification of RSC96 intercellular GSH level in different groups. (G) The ROS level in rats’ DRG tissue was detected by ELISA kit. (H) Comparison of GSH level in rat DRG tissues among different groups. The results are presented as mean ± SEM, n = 3. #p < 0.05, ##p < 0.01, ###p < 0.001, and ####p < 0.0001 vs. the control group. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001 vs. the model group.