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. 2021 Sep 24;12:716684. doi: 10.3389/fphar.2021.716684

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Copyright © 2021 Yang, Ma, Zhang, Sun, Zhang, Chang, Lin and Zhao.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Hig protected RSC96 against oxidative stress-induced apoptosis by regulating mitochondrial pathway. (A) △Ψm was detected by JC-1 probe using flow cytometer. The sum of quadrants H1-LL and H1-LR represented the depolarization extent of △Ψm. (B) Protein bands were detected by WB analysis. The ratios of Bcl-2/Bax protein level and the expression level of cyt-c protein were quantified. GAPDH served as an internal control. The results are presented as mean ± SEM, n = 3. ###p < 0.001 and ####p < 0.0001 vs. the control group. *p < 0.05, **p < 0.01, and ****p < 0.0001 vs. the t-BHP group.