Skip to main content
. 2021 Sep 24;12:669199. doi: 10.3389/fphar.2021.669199

FIGURE 4.

FIGURE 4

The role of TIPE2 in cell proliferation mediated by gracillin in BGC823 cells. (A) MTT assay for cell proliferation by TIPE2 and gracillin. 5 μM gracillin was used to treat the BGC823 cells and TIPE2 siRNA BGC823 cells for 48 h. (B) Colony formation for cell proliferation by TIPE2 and gracillin. 5 μM gracillin was used to treat the BGC823 cells and TIPE2 siRNA BGC823 cells for 7 days. (C) EdU staining for cell proliferation by TIPE2 and gracillin. 5 μM gracillin was used to treat the BGC823 cells and TIPE2 siRNA BGC823 cells for 12 h. (D) The quantitative analysis for EdU staining. (E) Western blot for the expression of TIPE2, AKT, and p-AKT. The values of the histogram are displayed as mean ± SD. 0.01 < p < 0.05 (*) represents a significant difference and 0.001 < p < 0.01 (**) represents a very significant difference to the normal control; 0.001 < p < 0.01 (##) represents a very significant difference and p < 0.001 (###) represents an extremely significant difference to the TIPE2 siRNA control.