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. 2021 Sep 24;12:669199. doi: 10.3389/fphar.2021.669199

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Copyright © 2021 Liu, Wang, Chen, Lin, Lin, Lin and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The role of TIPE2 in the cell cycle distribution mediated by gracillin in BGC823 cells. (A) Flow cytometry for cell cycle distribution by TIPE2 and gracillin. BGC823 cells and TIPE2 siRNA BGC823 cells were maintained with FBS-free medium for 36 h, then treated with 3 μM gracillin for another 24 h. (B) The quantitative analysis of cell cycle distribution by TIPE2 and gracillin. (C) Western blot for the expression of TIPE2, CDK1, p-CKD1, cyclin B1, and p21. (D) The quantitative analysis of protein expression. The values of the histogram were displayed as mean ± SD. 0.01 < p < 0.05 (*) represents a significant difference and 0.001 < p < 0.01 (**) represents a very significant difference to the normal control; 0.01 < p < 0.05 (^#) represents a significant difference, 0.001 < p < 0.01 (^##) represents a very significant difference and p < 0.001 (^###) represents an extremely significant difference to the TIPE2 siRNA control.