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. 2021 Sep 24;12:669199. doi: 10.3389/fphar.2021.669199

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Copyright © 2021 Liu, Wang, Chen, Lin, Lin, Lin and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The role of TIPE2 in the cell apoptosis mediated by gracillin in BGC823 cells. (A) Flow cytometer for cell apoptosis by TIPE2 and gracillin. BGC823 cells and TIPE2 siRNA BGC823 cells were treated with 3 μM gracillin for 24 h. (B) The quantitative analysis of cell apoptosis. (C) Elisa assay for the enzyme activities of caspase three and caspase 9. (D) Western blot for the expression of cleaved PARP, bcl2, cleaved caspase 9, and cleaved caspase 3. 0.01 < p < 0.05 (*) represents a significant difference, 0.001 < p < 0.001 (**) represents a very significant difference and p < 0.001 (***) represents an extremely significant difference to the normal control; 0.01 < p < 0.05 (^#) represents a significant difference, 0.001 < p < 0.01 (^##) represents a very significant difference and p < 0.001 (^###) represents an extremely significant difference to the TIPE2 siRNA control.