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. 2021 Sep 24;12:718380. doi: 10.3389/fimmu.2021.718380

Figure 1.

Figure 1

Poly (I:C) induces IFNλ1 expression and secretion by uterine epithelial cells. (A) ECC-1 uterine epithelial cells were stimulated with poly (I:C) for 3-24 hrs and IFNλ1 expression measured by RT-PCR. (B, C) Primary uterine epithelial cells were stimulated with poly (I:C) (25μg/ml) for 24 hrs prior to analysis of mRNA expression by real-time RT-PCR (B) and protein secretion (C) by ELISA. Each circle represents an individual patient (n = 34). (D) Percent increase in IFNλ1 secretion in the apical and basolateral compartments of transwell inserts following treatment of uterine epithelial cells with poly (I:C) (25μg/ml) for 24hrs versus untreated wells (n = 34). (E, F) Values for IFNλ1 secretion from panel E by poly (IC)-treated epithelial cells were subtracted from untreated wells to determine the difference in IFNλ1 secretion between matched poly (I:C)-treated and untreated wells (ΔIFNλ1 pg/ml) which was then plotted against patient age (E) (n = 34) or menopausal status (F) (n = 34). Each circle represents an individual patient. Data is shown as mean +/- SEM. Wilcoxon matched-pairs signed rank test (B–D). Non-parametric Spearman correlation analysis (E). Mann-Whitney non-parametric t-test (F). *p < 0.05; **p < 0.01; ****p < 0.0001.