Figure 8.

Estradiol (E₂) inhibits IFNλ1-induced upregulation of OAS2 and ISG15 in ECC-1 cells and uterine epithelial cells via ERα. (A) Polarized ECC-1 cells (n = 3) or (B) uterine epithelial cells (n = 3) were pretreated with Raloxifene (Rx) (5x10^-6M) for 1 hr prior to introduction of E₂ (5x10^-8M) for a subsequent 48 hrs. Following washout, Rx, E₂ or IFNλ1 (500 ng/ml) were added to the cells in the combinations listed for a further 24 hrs prior to mRNA analysis of OAS2 and ISG15 expression by real-time RT-PCR. mRNA expression data is presented as mean-fold +/- SEM change in gene expression over untreated control and is normalized to the housekeeping gene β-Actin, and then further normalized to the untreated control whose value is set to 1. Wilcoxon matched-pairs signed rank test. *p < 0.05.