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. 2021 Sep 24;12:741038. doi: 10.3389/fphys.2021.741038

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Copyright © 2021 Zhao, Cholewa, Shang, Yang, Ding, Liu, Xia, Zanchi and Wang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Effects of exercise on protein expression with relation to leucine-sensing. (A) blotting, (B) phosphorylation state of 4E-BP1 (Thr37/46), (C) phosphorylation state of p70S6K (Thr389), (D) phosphorylation state of mTOR (Ser2448), (E) relative expression of LeuRS and (F) relative expression of Sestrin2. SC, sedentary control group; AE, aerobic exercise training group; RE, resistance training exercise group. Data were expressed relative to the SC group. The expression of LeuRS and Sestrin2 were normalized to GAPDH. The phosphorylation state of mTOR signaling was calculated as the ratio of phosphorylated protein to total. ^**P < 0.01 vs. the SC group; ^##P < 0.01 vs. the AE group.