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. 2021 Sep 24;14:726331. doi: 10.3389/fnmol.2021.726331

FIGURE 2.

FIGURE 2

Distribution of pan-Akt, pT308-Akt and pS473-Akt. (A) Top panel shows an example of an axon stained with a pan-Akt antibody. The distribution is uniform. (B) Graph showing the mean staining intensity of pan-Akt staining in axons (n = 42) in segments devoid of mitochondria and those populated by mitochondria. An example of an axon stained with anti-pan-Akt and containing labeled mitochondria is shown below the graph. (C) Example of pT308-Akt staining along an axon treated with NGF for 10 min. The top panel shows a phase contrast image of the axon. (D) Example of pS473-Akt staining along an axon treated with NGF for 10 min. The top panel shows a phase contrast image of the axon with overlaid pS473-Akt. The scale bar in (D) also applies to (A,C).