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. 2021 Sep 24;9:666303. doi: 10.3389/fcell.2021.666303

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Copyright © 2021 Tan, Zhang, Li, Zhan, Qiao, Wu, Sun, Huang, Zhang, Zhang, Li, Li and Pan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Secretion deficiencies in the oviducts of Lgr4 KO mice. (A) A schematic showing the procedure for mouse oviduct epithelial cell–oocyte co-culture system. (B) Immunofluorescence analysis of cytokeratin 18 (KRT-18) and alpha-tubulin (Ac-Tub) to the mouse oviduct epithelial cells. (C) Immunofluorescence analysis of PAX8. (D) The average percentage of the MII oocytes in response to different treatments. (E) The released protein level of OVGP1. (F) The released protein level of IL-8. (G) The periodic acid–Schiff (PAS) staining between the WT and Lgr4 knockout oviduct. (H) The Alcian blue staining between the WT and Lgr4 knockout oviduct. ^∗p ≤ 0.05; ^∗∗p ≤ 0.01. Scale bar, 10 μm in (B,C), 25 μm in (G,H). At least three independent experiments were carried out.