Figure 2.

Effect of capsaicin and LPS treatment on gene expression and secretion of cytokines in IPEC-J2 cells. After being pretreated with capsaicin (100 μM) for 2 h, IPEC-J2 cells were stimulated with LPS (10 μg/mL) for 2 h (for real time-PCR assay) or 6 h (for ELISA assay). Total RNA was extracted from cells and the mRNA abundance of TNF-α (A), IL-8 (B), IL-1β (C), IL-10 (D), TNF-α secretion (E), and IL-8 secretion (F) was detected by real time-PCR as described in the Materials and Methods. The level of IL-8 and TNF-α in supernatants were measured as described in the Materials and Methods’ Values are presented as mean ± SEM. Different superscript letters on bars (a, b) indicate significant mean differences, P < 0.05.