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Effects of capsaicin and LPS treatment on both gene and protein expression of tight junction proteins in IPEC-J2 cells. Cells were cultured and treated using the same condition as Figures 2, 4. The mRNA abundance of ZO-1 (A), OCLN (B), CLDN3 (C), protein expression of ZO-1 (D,F), and OCLN (E,G) were determined by real time-PCR and Western blotting, respectively. Values are mean ± SEM. Different letters (a, b) on bars indicate significant mean difference, P < 0.05.
