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. 2021 Sep 24;11:728047. doi: 10.3389/fonc.2021.728047

Figure 3.

Figure 3

Blockage of NF-кB activation reverses DPP-4i-driven metastasis in vitro. (A–C) NF-кB inhibitor reverses DPP-4i-driven NF-кB activation. 4T1 cells were co-treated with Sax (0.4 μM) or Sit (1.2 μM) and NF-кB inhibitor BAY 11-7082 (0, 2, and 4 μM), respectively. (A) Total p65 and p-p65 expressions and (B, C) NF-кB-responsive genes were detected by Western blotting. (D, E) NF-кB blockage attenuates DPP-4i-driven cell migration and invasion in vitro. 4T1 cells were subject to (D) cell migration and (E) cell invasion upon co-treatment with Sax (0.4 μM) or Sit (1.2 μM) and BAY 11-7082 (0, 0.1, and 0.25 μM) for 24 h. Migration or invasion cells were counted in 5–10 random fields. Data are presented as mean ± SD of three independent experiments. Representative images are shown. *p < 0.05, **p < 0.01, and ***p < 0.001 between the indicated groups determined by unpaired Student’s t-test or the one-way analysis of variance (ANOVA).