Table 2.
Parameters of ACAD9 and its complexes calculated from the SEC-SAXS data
| Samples | Rg(nm) | Dmax(nm) | Vporod(nm3) | MW (kDa) By sequence |
MW (kDa) By HPLC |
MW (kDa)a By SAXS |
|---|---|---|---|---|---|---|
| ACAD9 | 3.41 | 10.3 | 230 | 130 | 110 | 140 |
| ACAD9/ECSIT | 5.02 | 16.0 | 494 | 223 | 270 | 320 |
| ACAD9/ECSIT/His6-SMT3-NDUFAF1 | 6.78 | 21.3 | 1340 | 637 | 620 | 710 |
The abbreviations used are as follows: Rg, radius of gyration; Dmax, maximum size of the particle; VP, excluded volume of the hydrated particle, Experimental mass, experimental molecular mass of the solute.
a
SAXS MW estimates were obtained from the Primus program according to Bayesian Inference (Konarev et al., 2003). See also Figure S4.