REAGENT or RESOURCE	SOURCE	IDENTIFIER
Bacterial and virus strains
BL21(DE3)	Thermo Fisher Scientific (Rockford, IL)	EC0114
Chemicals, peptides, and recombinant proteins
Ubiquitin like protease (ULP)	Sigma-Aldrich (St. Louis, MO)	SAE0067
Thrombin	Sigma-Aldrich (St. Louis, MO)
Palmitoyl-coenzyme A	Avanti Polar Lipids, Inc. (Alabaster, AL)
Restriction enzymes, DNA polymerases, ligase, DNA, and protein markers	Thermo Fisher Scientific (Rockford, IL)
Ni-NTA resin	Clontech Laboratories (Mountain View, CA)
Deposited data
SMT3 structure	PDB: 3PGE
VLCAD structure	PDB: 3B96
MCAD-ETF structure	PDB: 2A1T
Oligonucleotides
5′-TAATACGACTCACTATAGGG-3′	This work	T7 promoter
5′-CCCGGC GTCGAC TCA GTG ATG ATG-GTG GTG ATG GCT CCC GCA GGT GCG GTC CAG AGG-3′	This work	3′ antisense
Recombinant DNA
ECSIT (UniProtKB-Q9BQ95) cDNA	GenScript (Piscataway, USA)
NDUFAF1 (UniProtKB-Q9Y375) cDNA	GenScript (Piscataway, USA)
pET21b vector	Sigma Aldrich	69741
pET28a vector	Sigma Aldrich	69864
His6-SMT3-pET28a vector
QuickChange Site-Directed Mutagenesis Kit	Agilent	200519
Software and algorithms
Protein Calculator v3.4		http://protcalc.sourceforge.net/
ATSAS	(Franke et al., 2017)	https://www.embl-hamburg.de/biosaxs/software.html
PRIMUS	(Konarev et al., 2003)	https://www.embl-hamburg.de/biosaxs/software.html
GNOM	(Svergun, 1992)	https://www.embl-hamburg.de/biosaxs/software.html
DAMMIF	(Franke and Svergun, 2009)	https://www.embl-hamburg.de/biosaxs/software.html
DAMSTART	(Petoukhov et al., 2012)	https://www.embl-hamburg.de/biosaxs/software.html
DAMMIN	(Svergun, 1999)	https://www.embl-hamburg.de/biosaxs/software.html
SWISS-MODEL server	(Waterhouse et al., 2018)	https://swissmodel.expasy.org/
Robetta web server	(Kim et al., 2004)	https://robetta.bakerlab.org/
GalaxyGemini server	(Lee et al., 2013)	http://galaxy.seoklab.org/cgi-bin/submit.cgi?type=GEMINI
SUPCOMB	(Kozin and Svergun, 2001)	https://www.embl-hamburg.de/biosaxs/software.html
UCSF Chimera	(Pettersen et al., 2004)	https://www.cgl.ucsf.edu/chimera/
FoXS server	(Schneidman-Duhovny et al., 2013; Svergun, 1999)	https://modbase.compbio.ucsf.edu/foxs/
Phyre2	(Kelley et al., 2015)	http://www.sbg.bio.ic.ac.uk/phyre2/html/page.cgi?id=index
Graphpad PRISM		https://www.graphpad.com/company/