FIGURE 1.

Molecular modeling of novel GlyR β subunit mutants. (A) Alignment of GlyR subunits α1, α2, α3, β subunits from human and the β subunit variants concentrating on transmembrane segments. Numbers of amino acid residues in mutant variants refer to non-mature protein, (SP) signal peptide. (B–D) Cartoon representation of the 3α:2β glycine receptor heteropentamer homology model viewed from extracellular domain (B), from the intracellular side (C) and the membrane plane (D). GlyR α-subunits are colored orange and yellow and β-subunits are colored in green. (E) Close-up views of the interaction of residues in WT (left panels) and the mutant (right panels) model. All critical residues are shown as ball and stick, whereas the backbone is shown in cartoon representation, numbering refers to mature protein.