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. 2021 Sep 24;12:719689. doi: 10.3389/fmicb.2021.719689

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Copyright © 2021 Wysocka, Jagielska, Łężniak and Sabała.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SpM23_A is produced under native conditions. Western blot analysis showing the presence of the SpM23_A protein identified in Staphylococcus pettenkoferi strains. EXC: extracellular fraction (concentrated 25-fold); CWP: LiCl-extracted cell wall protein fraction (concentrated 100-fold); CMP: SDS-extracted cell membrane protein fraction (concentrated 100-fold). Bioactivity assay of SpM23_A produced by S. pettenkoferi strains or spotted solution of recombinant enzyme (0.1μg, positive control). A S. aureus ΔtagO lawn was used as the biomarker owing to its susceptibility to SpM23_A in conditions of high ionic strength, such as in TSB medium. For additional controls, see Supplementary Figure 6.