FIGURE 1.

Characterization of Tmm₇₂₁₁. (A) SDS-PAGE analysis of the purified Tmm₇₂₁₁ protein. (B) Absorbance spectra of Tmm₇₂₁₁. Black line, the absorbance spectrum of the purified Tmm₇₂₁₁. Red line, the absorbance spectrum of the purified Tmm₇₂₁₁ with the addition of equimolar amount of NADPH (0.1 mM) under aerobic conditions. (C) HPLC assay of the enzymatic activity of the recombinant Tmm₇₂₁₁ on DMS. The peaks of DMSO and NADP⁺ monitored at 210 nm were indicated with black and red arrows, respectively. The reaction system without Tmm₇₂₁₁ was used as the control. The DMSO (1.25 mM) and NADP⁺ (0.4 mM) standards were used as positive controls. (D) Effect of temperature on the enzymatic activity of Tmm₇₂₁₁. (E) Effect of pH on the enzymatic activity of Tmm₇₂₁₁. The optimal pH was examined at 25°C using Bis-Tris buffer for pH 6–7, Tris buffer for pH 7–9 and glycine buffer for pH 9–10. The standard errors are from three independent experiments.