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Kinetic parameters for Tmm7211 and its mutants toward NADPH.
| Enzyme | apparent KM (μM) | kcat (min–1) |
| Wild type | 12.8 ± 0.4 | 4.5 ± 0.3 |
| Asn72Ala | 34.1 ± 1.0 | 1.9 ± 0.2 |
| Ser203Ala | 134.8 ± 11.1 | 1.0 ± 0.1 |
| Arg226Ala | 101.8 ± 8.6 | 0.9 ± 0.1 |
| His227Ala | 41.2 ± 2.3 | 2.5 ± 0.2 |
| Asn288Ala | 61.1 ± 4.0 | 1.3 ± 0.1 |
| Arg409Ala | 44.9 ± 5.6 | 2.7 ± 0.1 |
| Asp314Ala | 32.0 ± 0.6 | 2.1 ± 0.1 |
| Asp314Glu | 9.7 ± 0.5 | 5.2 ± 0.2 |
