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Specificity of the PCR primers. Comparison of nucleotide sequences of the 16S rRNA genes corresponding to primers Bps16S-42L (A) and Bps16S-427R (B). The nucleotide sequence of Ara− B. pseudomallei isolates was compared with those of Ara+ B. pseudomallei isolates, other Burkholderia spp., H. seropedicae, R. eutropha, P. aeruginosa, P. putida, S. typhi, and E. coli. The sequences at the primer sites are underlined.
