Ethidium bromide-stained agarose gel electrophoresis of the amplified 16S rRNA genes of Ara− and Ara+ B. pseudomallei isolates performed by using the multiplex primer set. M, 100-bp marker; lane 1, Ara− B. pseudomallei isolate K94050; lane 2, Ara− B. pseudomallei isolate K96243; lane 3, Ara+ B. pseudomallei isolate S95019; lane 4, Ara+ B. pseudomallei isolate TRF681; lane 5, negative control.