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. 2021 Sep 2;297(4):101162. doi: 10.1016/j.jbc.2021.101162

Figure 3.

Figure 3

ABCG2 is upregulated in CDK7i-resistant cells and correlates with worse overall survival in TNBC.A, Gene Set Enrichment Analysis (GSEA) of upregulated genes using the ABC transporter gene signature from Kyoto Encyclopedia of Genes and Genomes (KEGG) (23) in the CDK7i-sensitive/parental and resistant MDA-MB-468 and MDA-MB-231 cells. B, heatmap of the upregulated ABC transporter genes identified using GSEA. C and D, verification of changes in ABCG2 mRNA (C) and protein (D) expression in sensitive/parental and resistant MDA-MB-468 and MDA-MB-231 cells. Quantitation of ABCG2 expression in MDA-MB-468 and MDA-MB-231 cells are from three independent experiments. Bars are means ± SD. Results are expressed relative to total protein. ∗p < 0.05. E, overall survival curves in patients with basal (predominant subtype of TNBC) breast cancer generated with KM Plotter that were stratified based upon the expression of ABCG2 (high = 83 patients, low = 158 patients).