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. 2021 Sep 2;297(4):101162. doi: 10.1016/j.jbc.2021.101162

Figure 5.

Figure 5

Epithelial to mesenchymal transition (EMT) signature genes are upregulated in CDK7i-resistant TNBC cells.A, phase contrast images of sensitive/parental (MDA-MB-468 and MDA-MB-231) and resistant (MDA-MB-468R and MDA-MB-231R) cells. The scale bar represents 30 μm. B, Gene Set Enrichment Analysis of upregulated genes associated with EMT (Hallmark v7.4) is shown for the MDA-MB-468R and C, MDA-MB-231R cells. D–G, quantitative RT-PCR confirmation of the upregulation of SNAI1 (D), ITGB4 (E), FN1 (F), and SNAI2 (G) with the acquisition of CDK7i resistance. H–J, expression of EMT-associated proteins SNAI1 (H), ITGB4 (I), and FN1 (J) in MDA-MB-468 and MDA-MB-231-sensitive/parental and CDK7i-resistant cells. Cell line data are the results of three independent experiments. Bars are means ± SD. Protein results are expressed relative to the total protein. ∗p < 0.05.