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. 2021 Sep 24;12:637317. doi: 10.3389/fendo.2021.637317

Table 2.

Differential metabolites identified in POS and NEG models.

MS2 name RT (min) m/z Relative contents VIP p value Q value FC
PN PO
POS mode
Upregulated PC (16:0/16:0) 0.76 756.53 0.0057 0.0098 1.05 0.03 0.09 1.72
Pseudouridine 4.72 245.08 0.1490 0.2238 1.49 <0.001 0.02 1.50
l-arginine 8.65 175.12 15.1287 22.6698 2.05 <0.001 <0.001 1.50
α-d-Glucose 1-phosphate 4.72 261.05 0.0402 0.0561 1.33 0.02 0.05 1.40
d-Ornithine 8.31 133.10 0.1118 0.1537 1.11 0.01 0.03 1.38
Downregulated Dihydrolipoate 6.30 226.08 0.9609 0.8210 1.48 0.02 0.06 0.85
l-Proline 5.09 157.10 0.9086 0.7393 1.32 0.04 0.10 0.81
Ile-Ala 5.98 241.10 0.0164 0.0131 1.43 0.01 0.05 0.79
l-Pyroglutamic acid 5.31 147.08 0.0820 0.0641 1.77 <0.001 0.02 0.78
3-Mercapto-2-butanone 4.31 209.07 0.0063 0.0048 1.68 <0.001 0.02 0.76
l-Pipecolic acid 4.31 171.11 3.8816 2.8309 1.91 <0.001 0.01 0.73
His-Met 5.07 304.15 0.0105 0.0077 2.20 <0.001 <0.001 0.73
Xanthylic acid (XMP) 2.80 364.05 0.0059 0.0037 1.35 <0.001 0.01 0.63
N-Acetylglutamine 5.07 189.09 0.1214 0.0749 2.10 <0.001 <0.001 0.62
Glutaric acid 5.52 282.11 0.1437 0.0794 1.75 0.02 0.06 0.55
Leu-Leu 2.62 527.32 0.0099 0.0055 1.28 0.02 0.06 0.55
Gly-Glu 5.44 246.11 0.0375 0.0190 1.62 0.02 0.07 0.51
Myristoleic acid 2.22 226.18 0.0238 0.0119 1.12 0.01 0.03 0.50
d-Glucuronate 4.91 255.07 0.0249 0.0092 1.92 0.01 0.05 0.37
NEG mode
Upregulated Thymine 4.96 185.06 0.2174 0.7567 1.79 <0.001 0.08 3.48
hydrocortisone 21-acetate 0.73 425.20 0.0458 0.0865 2.04 0.02 0.13 1.89
Dihydrothymine 7.04 165.01 0.2288 0.4273 1.41 <0.001 0.03 1.87
l-Threonate 6.19 195.05 0.3783 0.6984 1.06 0.01 0.11 1.85
l-Glutamate 6.30 146.05 0.5866 1.0429 1.65 <0.001 0.07 1.78
Atrolactic acid 1.73 165.06 0.0215 0.0333 2.53 <0.001 0.06 1.55
Xanthine 3.45 151.03 0.4823 0.7027 1.14 0.05 0.19 1.46
Myristic acid 0.73 227.20 0.0257 0.0372 2.35 <0.001 0.04 1.45
l-Valine 5.56 116.07 0.0715 0.1024 2.00 <0.001 0.05 1.43
Azelaic acid 5.42 187.10 0.0322 0.0434 1.15 0.04 0.18 1.35
Indoleacetic acid 2.36 174.06 0.0197 0.0259 1.32 0.05 0.19 1.32
l-Leucine 4.97 130.09 0.1478 0.1938 1.86 0.01 0.12 1.31
3,4-Dihydroxymandelic acid 5.50 183.03 0.0306 0.0399 1.68 0.01 0.09 1.30
Palmitic acid 0.72 255.23 0.5133 0.6636 2.28 <0.001 0.03 1.29
5-Methoxyindoleacetate 5.57 226.04 0.0024 0.0031 1.21 0.01 0.12 1.28
dl-lactate 3.67 89.03 14.6582 18.5742 1.39 0.01 0.10 1.27
Indolelactic acid 2.72 204.07 0.2556 0.3184 1.96 0.04 0.18 1.25
l-Tyrosine 4.86 180.07 1.0350 1.2788 1.43 0.02 0.12 1.24
l-Alanine 5.56 88.04 0.5023 0.6196 1.51 0.01 0.12 1.23
N-acetyl-l-tyrosine 3.53 222.08 0.0952 0.1098 1.58 0.03 0.16 1.15
Downregulated α-N-acetyl-l-glutamine 4.95 187.07 0.6836 0.5187 2.65 <0.001 0.03 0.76
3-Hydroxycapric acid 0.77 169.12 0.0178 0.0133 1.09 0.05 0.19 0.75
l-Histidinol phosphate 4.75 280.06 0.0075 0.0054 1.81 0.02 0.12 0.72
2-Deoxyribose 5-phosphate 2.69 213.02 2.0023 1.3580 1.58 0.05 0.19 0.68
N-acetyl-d-galactosamine 1.74 242.18 0.0312 0.0205 1.25 0.05 0.19 0.66
l-Gulonic gamma-lactone 3.97 237.06 0.0532 0.0160 2.69 <0.001 0.05 0.30
Fructose 1-phosphate 0.37 259.03 0.3357 0.0406 3.28 <0.001 0.01 0.12

MS, mass spectrometry; m/z, mass-to-charge ratio; NEG, negative ion; PN, perimenopausal women without obesity; PO, perimenopausal women with obesity; POS, positive ion; RT, retention time; VIP, variable importance in projection.

The relative content of each metabolite was calculated by normalization of the contents with the internal standard.