Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Sep 24;12:738481. doi: 10.3389/fimmu.2021.738481

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2021 Palmroth, Kuuliala, Peltomaa, Virtanen, Kuuliala, Kurttila, Kinnunen, Leirisalo-Repo, Silvennoinen and Isomäki

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

(A) Graphical overview of the study and (B–I). Flow cytometry gating strategy and examples of cytokine-induced phosphorylated STAT (pSTAT) in each cell population in one patient sample. (B) Monocyte gate was set based on CD33 positivity and light scattering characteristics (SSC-A). (C) Lymphocytes were gated based on light scattering characteristics (FSC-A and SSC-A). (D) Among lymphocytes, T cell gates were set to comprise CD3⁺CD4⁺ or CD3⁺CD4^- populations. (E) Among lymphocytes, B cell gate was set to comprise CD3^- and CD20⁺ population. (F) pSTAT1 histograms in unstimulated (open peak) and IFN-γ-stimulated (grey peak) monocytes. (G) pSTAT3 histograms in unstimulated (open peak) and IL-10-stimulated (grey peak) CD4⁺ T cells. (H) pSTAT5 histograms in unstimulated (open peak) and IL-15-stimulated (grey peak) CD4^- T cells. (I) pSTAT6 histograms in unstimulated (open peak) and IL-4 stimulated (grey peak) B cells. IFN, interferon; IL, interleukin, (p)STAT, (phosphorylated) signal transducer and activator of transcription.