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. 2021 Oct 8;37(4):109881. doi: 10.1016/j.celrep.2021.109881

Figure 2.

Figure 2

2B04 and 2H04 target distinct epitopes on the RBD

(A) 2B04 targets an epitope centered on the RBM of the SARS-CoV-2 RBD. Complementarity-determining regions (CDRs) of 2B04 are shown as cartoon ribbons, with the heavy chain colored cyan and the light chain colored pale turquoise. The RBM is shown as a transparent surface with underlying ribbon diagram.

(B) Distribution of 2B04 heavy- versus light-chain contacts on the surface of the RBD. Heavy-chain contacts colored as in (A).

(C) 2H04 targets an epitope adjacent to the RBM on the SARS-CoV-2 RBD, distal from the RBM loop. CDR regions of 2H04 are shown as cartoon ribbons, with the heavy chain colored magenta and the light chain colored violet.

(D) Distribution of 2H04 heavy- versus light-chain contacts on the surface of the RBD. Contacts colored as in (C).

(E) Alignment of 2B04 and 2H04 models to the 2B04 RBD. 2B04 contacts are colored as in (A), and 2H04 contacts are colored as in (C). N450, the only shared contact between 2B04 and 2H04, is colored in blue on the RBD.

(F) BLI analysis of RBD mutants to 2B04, 2H04, and ACE2. Results represent mean (±standard deviation [SD]) from two independent experiments.