wt NF-Y associates a nucleosome-bound Ea Y box. (A) EMSA of a dose response of wt NF-Y on mock-reconstituted (0.5, 1, 3, and 10 ng; lanes 1 to 4) or nucleosome (NUC.)-reconstituted (lanes 6 to 9) Ea fragment 2 (−115 to +60 of Ea [36]). Lane 5, no NF-Y added to nucleosomal DNA. (B) A 70% nucleosomized Ea fragment 2 was run without NF-Y (lane 1), with 5 ng of NF-Y (lane 2), and with the same amount of NF-Y incubated with the indicated anti-NF-Y antibody (Ab; 200 ng of purified antibody; lanes 3 to 5). The same was in lanes 7 to 10 except that mock-reconstituted DNA was used.