FIG. 2.

Binding of NF-Y during nucleosome assembly. Stoichiometric amounts of histones were assembled in high salts with Ea fragment 2 and cold competitor DNA, in the absence (lanes 1 to 6) or presence (lanes 7 to 12) of 5 ng of wt NF-Y. In lanes 13 to 18, 5 ng of wt NF-Y was used as in lanes 7 to 12, in the absence of histones. Mixtures were progressively diluted, and at each NaCl concentration, aliquots were added to a running polyacrylamide gel. The NF-Y, H3-H4, nucleosome, and NF-Y–nucleosome bands are indicated.