FIGURE 3.

A comparative genome analysis for Lactobacillaceae based on the complete genome sequence of the newly developed L. acidophilus strain. (A) Neighbor-joining tree based on 16S rRNA sequences for 24 Lactobacillaceae families including the strain newly developed in this study. Bootstrapping was performed 1,000 times, and Bacillus subtilis 168 was considered as an outgroup. (B) Hierarchical clustering results based on average nucleotide identity among 24 Lactobacillaceae families. (C) A comparison of predictive SEED ratios between 24 Lactobacillaceae strains. (D) SEEDs with statistically significant differences between L. acidophilus and the rest of the Lactobacillaceae strains at 5% significance level after multiple testing adjustments. *P < 0.01 and **P < 0.001 in comparison of L. acidophilus and other strains.