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. 2021 Aug 7;8(19):2101848. doi: 10.1002/advs.202101848

Figure 4.

Figure 4

OGP suppresses CSCs via the YGFGG domain that can be cleaved by CD10. A–E) Indicated tumor cell lines after prolonged mammosphere culture were treated with 5× 10−9 m OGP1–14, OGP10–14, or OGP1–9, which is approximately equivalent to the OGP concentration of 10% FBS used in conventional cell culture. A,B) Representative images of mammosphere formation in A) MCF‐7mammo cells and B) BT‐474mammo cells. Scale bar, 100 µm. C,D) Percentage of ALDH1+ cells in C) MCF‐7mammo cells and D) BT‐549mammo cells (mean ± SEM, n = 3). E) Percentage of CD44highCD24low cells in MCF‐7mammo cells (mean ± SEM, n = 3). F–J) Indicated tumor cell lines after prolonged mammosphere culture were treated with 5 × 10−9 m OGP1–14 with or without 5 µg mL−1 rhCD10. F,G) Representative images of mammosphere formation in F) MCF‐7mammo cells and G) BT‐474mammo cells. Scale bar, 100 µm. H,I) Percentage of ALDH1+ cells in H) MCF‐7mammo cells and I) BT‐549mammo cells (mean ± SEM, n = 3). J) Percentage of CD44highCD24low cells in MCF‐7mammo cells (mean ± SEM, n = 3).