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. 2021 Aug 11;8(19):2101691. doi: 10.1002/advs.202101691

Figure 6.

Figure 6

Histology, viability, and endothelial morphology of the kidney after experimental procedures. A1–E1, A4–E4) H&E photomicrographs to show renal cortex and medulla respectively, comparing morphological and architectural changes with CPA (VS55) perfusion, vitrification and washout following vitrification and nanowarming to fresh control and cryopreserved negative control sections (20×). A2–E2, A5–E5) Confocal microscopy shows vascular endothelial (CD31) labeling of kidney cortex and medulla and demonstrates changes to vascular endothelium across treatment groups (20×). A3–E3, A6–E6) Merged confocal microscopy of kidney cortex and medulla respectively, labeling vascular endothelium (CD31, red), nuclei (DAPI, blue), and d‐galactosyl residues of n‐acetyl‐d‐galactosamine end groups (Isolectin GS‐IB4, cyan) to demonstrate nuclear, vascular endothelium and tubular luminal alterations following preservation and rewarming (20×). A7–E7) Confocal microscopy demonstrates viability of kidney cortex by labeling live cells (Acridine Orange, cyan) and dead cells (Propidium Iodide, red) to assess viability at the center of a 500 µm thick kidney slice prepared from whole organs after each of the treatments (20×). Scale bar is 100 µm for confocal microscopy images (CD31 and AO/PI) (A2–E3, A5––E7) and 150 µm for histology images (A1–E1,A4–E4).