Figure 3.

Principle validations of O₂ release for mitigating hypoxia and enhancing ROS, and ROS conversion into RNS. a) Typical FCM patterns (right) and quantitative fluorescence intensities (left) of hypoxic MCF‐7 cells after incubating with different samples (dose: 200 µg mL⁻¹ FHMON) labeled by FITC for evaluating their engulfment by hypoxic MCF‐7 cells. b) FIM images of hypoxic MCF‐7 cells that experienced different treatments after hypoxia probe (Hypoxyprobe Green) staining for tracking hypoxia alleviation, and scale bar = 50 µm. c) FIM images of hypoxic MCF‐7 cells that experienced different treatments after ROS probe (DCFH‐DA) and RNS probe (DAF‐AM DA) staining which are used monitor ROS and RNS levels, respectively, and scale bar = 100 µm. d,e) Quantitative fluorescence intensities of ROS and RNS in hypoxic MCF‐7 cells obtained according to image (c). f,g) Expression levels of different proteins in hypoxic MCF‐7 cells that experienced different treatments via WB analysis. Note, NIR parameter: 660 nm, 0.1 W cm⁻², pulsed irradiation for 5 min in total with 5 cycles and 1 min interval between two cycles. Data are expressed as mean ± SD (n = 3). Statistical analyses were performed using a Student's t‐test in Prism software, and *P ˂ 0.05, **P ˂ 0.01, and ***P ˂ 0.001.