Figure 2.

Neutrophils were pivotal for bone‐healing at the initiating stage. α‐Ly6G or α‐F4/80 antibody was continuously injected from 2 days pre‐ to 7 days post‐cranial defect creation for depletion of neutrophils or macrophages (referred to as N‐ and M‐), respectively, with IgG antibody as a control. A) Micro‐CT reconstruction of the defect site after 90 days. Quantification histogram of B) bone volume/total volume (BV/TV), C) bone mineral density (BMD), D) trabecular thickness (Tb.Th.), E) trabecular space (Tb.Sp.), and F) trabecular number (Tb.N.). (** p < 0.01, * p < 0.05; n = 3 for each group)