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. 1999 Jun;37(6):1941–1947. doi: 10.1128/jcm.37.6.1941-1947.1999

FIG. 2.

FIG. 2

FIG. 2

FIG. 2

Fluorescent probe assay for detection of HSV DNA. ΔRn corresponds to the increase in reporter dye intensity relative to the passive internal reference. (A) Detection of gB amplicon with the gB forward and gB reverse primers and the gB type-common primer. The results are for triple replicates of serial 10-fold dilutions of gB amplicon DNA. The negative control contains the PCR master mixture with unrelated DNA. The shaded area delineates the negative threshold. (B) Results for three representative genital tract samples. Specimen A contained 108 copies of HSV DNA, specimen B contained 104 copies of HSV DNA, and specimen C contained 45 copies of HSV DNA. (C) Results for 17 duplicate clinical samples compared with the results on a standard curve for HSV amplicon DNA. The standard curve is linear between 101 and 108 copies of amplicon DNA. The correlation coefficient between the 17 samples and the standard curve is 0.998. Each sample was run in duplicate, and the results for both replicates are depicted.