Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2021 Oct 7;9(1):e002394. doi: 10.1136/bmjdrc-2021-002394

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© Author(s) (or their employer(s)) 2021. Re-use permitted under CC BY-NC. No commercial re-use. See rights and permissions. Published by BMJ.

This is an open access article distributed in accordance with the Creative Commons Attribution Non Commercial (CC BY-NC 4.0) license, which permits others to distribute, remix, adapt, build upon this work non-commercially, and license their derivative works on different terms, provided the original work is properly cited, appropriate credit is given, any changes made indicated, and the use is non-commercial. See: http://creativecommons.org/licenses/by-nc/4.0/.

PMC Copyright notice

Aspect of granulation state and size of MIN6 cells labeled with hIns-dsRed E5. (A) MIN6 cells labeled with hIns-dsRed E5 were cultured for 18 hours either in the presence of 500 µM tolbutamide (left) or 1 µM clonidine (right) or control cultured (middle) and imaged by SD-CLSM close to the plasma membrane (0.2 µm, upper row) or close to the cell middle (4 µm, lower row). Note the considerable mistargeting and heterogeneous appearance. The length of the bars is 10 µm. (B) In the TIRF mode, a clear identification of single granules was possible after culture in the presence of tolbutamide or clonidine or after control culture. The different ratio of green to red fluorescence is clearly visible. Note the presence of red granules in the submembrane space. SD-CLSM, spinning disk confocal laser scanning microscopy.