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. 2021 Oct 8;19:222. doi: 10.1186/s12915-021-01157-3

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 6 — Conservation of Kr-h1 phosphorylation in other insects. A Western blot showing Kr-h1 phosphorylation in penultimate instar larvae of Bombyx mori, Tribolium castaneum, and Drosophila melanogaster, respectively. B Luciferase reporter assays after co-transfection of pGL4.10-4×BmE93^{-2844 to -2827} with pAc5.1/Flag-BmKr-h1, pAc5.1/Flag-BmKr-h1^S76A, pAc5.1/Flag-BmKr-h1^S76D or pAc5.1/Flag vector control into S2 cells. Methoprene was applied at 10 μM. C Luciferase reporter assays using S2 cells co-transfected with pGL4.10-4×TcE93 ^{-50 to -33} with pAc5.1/Flag-TcKr-h1, pAc5.1/Flag-TcKr-h1^S124A, pAc5.1/Flag-TcKr-h1^S124D, or pAc5.1/Flag. D Luciferase reporter assays using S2 cells co-transfected with pGL4.10-4×DmE93^{-2095 to -2078} with pAc5.1/Flag-DmKr-h1, pAc5.1/Flag-DmKr-h1^S255A, pAc5.1/Flag-DmKr-h1^S255D or pAc5.1/Flag. Means labeled with different letters indicate significant difference at P<0.05. n=3