Table 2.
EC50 values of PG-induced ILC2 migration and IL-5 and IL-13 cytokine secretion were calculated and are given as mean ± standard error of the mean (SEM), n = 3
| ILC2 migration and cytokine secretion EC50 [nM] | |||
|---|---|---|---|
| Metabolite | Migration | IL-5 secretion | IL-13 secretion |
| PGD2 | 17.4 ± 3.9 | 139.2 ± 15.5 | 131.9 ± 10.8 |
| DK-PGD2 | 14.2 ± 3.4 | 146.0 ± 18.0 | 159.2 ± 28.8 |
| ∆12-PGD2 | 19.3 ± 3.2 | 108.1 ± 11.8 | 125.2 ± 8.3 |
| 15-deoxy-∆12,14-PGD2 | 21.8 ± 6.3 | 175.4 ± 26.4 | 159.1 ± 13.9 |
| 9α,11β-PGF2 | –a | 526.9 ± 12,926.7 | 788.3 ± 173.7 |
| PGJ2 | 66.3 ± 7.0 | 244.4 ± 26.3 | 321.9 ± 43.8 |
| ∆12-PGJ2 | 91.7 ± 9.2 | 234.1 ± 30.6 | 343.2 ± 52.4 |
| 15-deoxy-∆12,14-PGJ2 | 38.1 ± 5.4 | 185.3 ± 21.5 | 226.2 ± 35.2 |
aCurve fit was ambiguous, EC50 value could not be determined